UC Riverside Scientists Isolate
Microorganisms That Break Down A Toxic Pesticide
Source: University Of California - Riverside
RIVERSIDE, Calif. - Feb. 27, 2003 - Scientists at the University
of California, Riverside report in the Journal of Environmental
Quality (JEQ) that they have isolated microorganisms capable of
degrading endosulfan, a chlorinated insecticide widely used all
over the world and which is currently registered to control insects
and mites on 60 U.S. crops. JEQ, established in 1972, is published
jointly by the American Society of Agronomy, Crop Science Society
of America, and the Soil Science Society of America.
Bioremediation of contaminated sites and water bodies by using
these microbial strains will provide an environment free of endosulfan
toxicity, the researchers argue in their paper. The research stands
to benefit the agrochemical industry and environmental agencies
involved in remediation of soil and water contaminated with organochlorine
pesticides. Currently, bioremediation is considered the most cost-effective
technology to remediate contaminants, including pesticides. The
usefulness of the new technology may be best measured economically
in soil and water quality impacted by pesticide spillage, overdosing,
and cleanup of agrochemical equipment.
Many health hazards are associated with endosulfan. Endosulfan
is a persistent organic pollutant or "POP" that enters
the air, water, and soil during its use and manufacture. Owing to
the persistence in the environment, residues of endosulfan can enter
the food chain and directly affect public health. Endosulfan's residues
have also been found in sediments and in surface and ground waters.
Endosulfan affects the central nervous system, kidney, liver, blood
chemistry and parathyroid gland and has reproductive, teratogenic
(causing birth defects) and mutagenic (causing genes to mutate more
frequently) effects.
"We have been successful in isolating strains that can use
endosulfan as a carbon and energy source," said William Frankenberger,
director of the UCR Center for Technology Development and professor
of soil science and soil microbiologist at UC Riverside. "Pollutants
are rapidly degraded by microorganisms when used as a carbon and
energy source. Out of 10 microorganisms isolated and screened for
their degradative capabilities towards endosulfan degradation, the
strains we isolated - Fusarium ventricosum and Pandoraea sp. - degraded
about 90% and 83% of 100 ppm endosulfan, respectively, in 15 days
using the pesticide as a carbon and energy source. Other bacterial
strains that we isolated using endosulfan as a sulfur source could
degrade about 70% endosulfan."
Total average annual use of endosulfan is estimated at approximately
1.38 million pounds of active ingredient. Classified as an organochlorine
(the same family of pesticide as DDT and dieldrin), endosulfan and
its breakdown products are persistent in the environment with an
estimated half-life of 9 months to 6 years. It is one of the most
commonly detected pesticides in U.S. water (38 states).
"In isolating these microbial strains, various environmental
samples were collected from different sites," said Frankenberger.
"Enrichment techniques were used to isolate microbial strains
which were capable of degrading endosulfan. The isolated microorganisms
were intensively screened for their degradative capabilities towards
endosulfan degradation, purified and identified by molecular tools."
The results of the study suggest that these strains are a valuable
source of endosulfan-degrading enzymes and may be used for the detoxification
of endosulfan in contaminated soils, wastedumps and water bodies,
as well as agricultural dealership sites, waste water from recycling
plants and unused or expired stockpiles of endosulfan.
This research was conducted in the Department of Environmental
Sciences at UC Riverside during 2001-2002. The department offers
B.S. and B.A. degrees in Environmental Sciences, and M.S. and Ph.D.
degrees in Soil and Water Sciences. The department is part of the
College of Natural and Agricultural Sciences. The forerunner of
the department was an agricultural chemistry research unit in the
world-renowned California Citrus Research Center and Agricultural
Experiment Station established in Riverside in 1907. |